Ceramic vessel 109. Sanctuary of Piedra del Águila (Orcera, Jaén, Spain)

Ceramic fragment 109 (possible the base of a pot) from UEC 4 of the Iberian sanctuary of Piedra del Águila (Orcera, Jaén, Spain). Wheelmade ceramic, oxidising firing. It has no decoration. The analysis of lipid contents was carried out using GC-MS and HPLC-APCI-MS, obtaining a lipid profile characteristic of ruminant animal fat.The analysis of lipid contents was carried out using GC-MS and HPLC-APCI-MS and GC-C-IRMS, obtaining a lipid profile indicative of a mixture of ruminant and non-ruminant animal fat. This conclusion is based on the identification of di and triacylglycerols (PP; PS; SS; PMP; PPP; PPO; PPMa?; PSP; SPO; SPS; SSO; SSS), in the P:S sn-2 index of 69/31, and ∆13C %3D 0.23.

Condition: New product

Dimensions

Alto : 7 Centimeters

Ancho : 2 Centimeters

Materials

pottery

Temporal

Period name : Iberians, Iberian

Date : 3rd ct. BC

Spatial

Location : Piedra del Águila

Geopolitical area : Orcera, Jaén, Spain

Spatial reference system : WGS84

Copyrights

Creative Commons - Attribution, Non-Commercial, No Derivatives (BY-NC-ND)

References

Article - Articulo - Rueda, C. y Bellón, J. P.(2016): "Culto y rito en cuevas: modelos territoriales de vivencia y experimentación de lo sagrado, más allá de la materialidad (ss. V-II a.n.e.)". ARYS, 14:43-80. https://doi.org/10.20318/arys.2017.3986

Digital Resources

Photo ceramic vessel 109 Instituto Universitario de Investigación en Arqueología Ibérica

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Photo.Interior of the sanctuary and deposition area of the ceramic vessel 109

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GC-MS del recipiente 109 Instituto Universitario de Investigación en Arqueología Ibérica

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Arquiberlab
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HPLC-APCI-MS ceramic vessel 109 Instituto Universitario de Investigación en Arqueología Ibérica

Creative Commons - Attribution, Non-Commercial, No Derivatives (BY-NC-ND)
Arquiberlab
http://creativecommons.org/licenses/by-nc-nd/3.0/

Activities

Archaeometric analysis

Chromatographic analysis and structural determination

Pottery. Content analysis

University Research Center for Iberian Archaeology

Gass Chromatography-Mass Spectrometry

Pottery. Lipids analysis

Destructive method for the identification of the total lipid profile: fatty acids, sterols, wax esters, fatty alcohols, triacylglycerols, etc. Any remains of soil were removed with an electric hand-drill. The sample was then grinded to the appropriate size in an agatha mortar (0.25 mm). Extraction with a the mixture chloroform/methanol (CHCl3:MeOH) (2:1 v/v) assisted by ultrasound. Derivatization of lipids to trimethylsilyl derivatives (TMS). The reaction tales place at 70 ºC for 30 min using N,O-bis-(trimethylsilyl) trifluoroacetamide (BSTFA) with 1 % trimethylchlorosilane (TMCS). The sample is solved in cyclohexane and then injected in GC-MS. Chromatographic separation is performed applying an adequate temperature programme.

Gas chromatography-Mass Spectrometry (GC-MS)

The analyses were performed using a using a gas chromatography equipment (model Thermo TraceGC Ultra) coupled to a Thermo DC Q II mass spectrometer. Autosampler Thermo Triplus (CICT, University of Jaén). The samples were introduced by on-column injection into a 15 m x 0.25 mm I.D. fused silica capillary column, coated with poly(dimethylsiloxane) stationary phase with 0.1 μm film thickness. Helium was used as the carrier gas (purity 99.99%)

High Performance Liquid Chromatography-Mass Spectrometry

Pottery. Analysis of triacylglycerols

Destructive method for the identification of TAGs content. Any remains of soil were removed with an electric hand-drill. The sample was then grinded to the appropriate size in an agatha mortar (0.25 mm). Extraction with a the mixture chloroform/methanol (CHCl3:MeOH) (2:1 v/v) assisted by ultrasound. Derivatization is no needed. Lipid extract is solved in isopropanol and then injected in HPLC-APCI-MS. Chromatographic separation is performed applying an adequate mobile phase gradient (mixture of isopropanol and methanol).

High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)

Agilent 1290 Infinity HPLC system connected to an Agilent 6220 TOF (time-of-flight) mass spectrometer equipped with APCI (Atmospheric Pressure Chemical Ionization) interface. (Departement of Physical and Analytical Chemistry, University of Jaén). Chromatographic column Zorbax Eclipse Plus C18 (150 mm x 4.6 mm, 1.8 μm particle size).