Vessel 119. Grave 231. Cemetery of La Noria (Fuente de Piedra, Málaga, Spain)

Plate. Grave 231 of burial mound H of the Iberian cemetery of La Noria (Fuente de Piedra, Málaga, Spain). Handmade pottery, oxidising atmosphere. It has no decoration. The analysis of lipid contents was carried out using GC-MS and HPLC-APCI-MS, obtaining a characteristic lipid profile of ruminant animal fat.

The interpretation is based on the identification of a set of chemical markers obtained after the joint application of these two techniques: volatile or short-chain fatty acids (C12: 0 and C14: 0), fatty acids with number odd carbon atoms and their corresponding chain isomers (C15: 0, i-C15: 0, C17: 0, i-C17: 0), positional isomer of oleic acid (i-C18: 1), where unsaturation (double bond) presents a different position in the hydrocarbon chain, TAGs formed mainly by saturated fatty acids (myristic: M, palmitic: P, stearic: S). The analysis by HPLC-APCI-MS determines a P/S index in sn-2 position of 55/45, indicative of ruminant animal fats.

Condition: New product

Dimensions

Alto : 7 Centimeters

Ancho : 24 Centimeters

Materials

pottery

Temporal

Period name : Iberians, Iberian

Date : Late 6th ct. BC-early 5th ct. BC

Spatial

Location : Cemetery of La Noria

Geopolitical area : Fuente de Piedra (Málaga, Spain)

Spatial reference system : WGS84

Copyrights

Creative Commons - Attribution, Non-Commercial, No Derivatives (BY-NC-ND)

References

Article - Andrino, M., Sánchez, P. J., Cumpían, A. y López, S. (2008):“Una necrópolis de incineración de las etapas iniciales de la Edad del Hierro en la comarca de Antequera. Resultados de la intervención arqueológica de urgencia en la parcela ur-6, Fuente de Piedra, Málaga”. Varia, 9. 359-378.

Chapter - Ruiz, A., Molinos, M., Cano, M.F., Montes, E., Ortuño, E. (2017): "El túmulo C de la necrópolis de la Noria (Fuente de Piedra). ¿La tumba de dos mujeres?", en: Ruiz, A. y Molinos, M. (Eds.), Catálogo de la exposición La Dama, el Príncipe, el Héroe y la Diosa. Conserjería de Cultura de la Junta de Andalucía, Sevilla, pp. 119-128.

Legal document - Resolución de 18 de noviembre de 2008, de la Dirección General de Bienes Culturales, por la que se incoa el procedimiento para la inscripción en el Catálogo General del Patrimonio Histórico Andaluz, como Bien de Interés Cultural, con la tipología de Zona Arqueológica, la necrópolis protohistórica de La Noria, en Fuente de Piedra, Málaga.

Digital Resources

Photo ceramic vessel 119 Instituto Universitario de Investigación en Arqueología Ibérica

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Photo of the cemetery of La Noria and locations of the burial mounds Instituto Universitario de Investigación en Arqueología Ibérica

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Photo of the burial mound H Instituto Universitario de Investigación en Arqueología Ibérica

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Arquiberlab
http://creativecommons.org/licenses/by-nc-nd/3.0/
GC-MS of the ceramic vessel 119 Instituto Universitario de Investigación en Arqueología Ibérica

Creative Commons - Attribution, Non-Commercial, No Derivatives (BY-NC-ND)
Arquiberlab
http://creativecommons.org/licenses/by-nc-nd/3.0/
HPLC-APCI-MS of the ceramic vessel 119 Instituto Universitario de Investigación en Arqueología Ibérica

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Arquiberlab
http://creativecommons.org/licenses/by-nc-nd/3.0/

Activities

Archaeometric analysis

Chromatographic analysis and structural determinarion

Pottery. Content analysis

University Research Center for Iberian Archaeology

Gass Chromatography-Mass Spectrometry

Pottery. Lipids analysis

Destructive method for the identification of the total lipid profile: fatty acids, sterols, wax esters, fatty alcohols, triacylglycerols, etc. Any remains of soil were removed with an electric hand-drill. The sample was then grinded to the appropriate size in an agatha mortar (0.25 mm). Extraction with a the mixture chloroform/methanol (CHCl3:MeOH) (2:1 v/v) assisted by ultrasound. Derivatization of lipids to trimethylsilyl derivatives (TMS). The reaction tales place at 70 ºC for 30 min using N,O-bis-(trimethylsilyl) trifluoroacetamide (BSTFA) with 1 % trimethylchlorosilane (TMCS). The sample is solved in cyclohexane and then injected in GC-MS. Chromatographic separation is performed applying an adequate temperature programme.

Gas Chromatography-Mass Spectrometry (GC-MS)

Gas chromatograph Thermo TraceGC Ultra coupled to a Thermo DC Q II mass spectrometer. Autosampler Thermo Triplus (CICT, University of Jaén) and equipped with a 15 m x 0.25 mm I.D. fused silica capillary column, coated with poly(dimethylsiloxane) stationary phase with 0.1 μm film thickness. Helium (He) is used as the carrier gas.

High Performance Liquid Chromatography-Mass Spectrometry

Pottery. Triacylglycerols analysis

Destructive method for the identification of TAGs content. Any remains of soil were removed with an electric hand-drill. The sample was then grinded to the appropriate size in an agatha mortar (0.25 mm). Extraction with a the mixture chloroform/methanol (CHCl3:MeOH) (2:1 v/v) assisted by ultrasound. Derivatization is no needed. Lipid extract is solved in isopropanol and then injected in HPLC-APCI-MS. Chromatographic separation is performed applying an adequate mobile phase gradient (mixture of isopropanol and methanol).

High Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Mass Spectrometry (HPLC-APCI-MS)

Agilent 1290 Infinity HPLC system connected to an Agilent 6220 TOF (time-of-flight) mass spectrometer equipped with APCI (Atmospheric Pressure Chemical Ionization) interface. (Departement of Physical and Analytical Chemistry, University of Jaén). Chromatographic column Zorbax Eclipse Plus C18 (150 mm x 4.6 mm, 1.8 μm particle size).